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[This article belongs to Volume - 70, Issue - 6]
Published on : 2025-06-17 14:41:40
Article Code: AMJ-17-06-2025-12320
Title : Phage Therapy Against Pseudomonas aeruginosa Biofilms in Cystic Fibrosis: A Personalized Bacteriophage CocktAIl Approach Guided by Host-Pathogen Genomics
Author(s) : Dr. Benjamin Foster, Dr. Amara Osei, Dr. Klaus Weber
Abstract :
Antibiotic resistance in Pseudomonas aeruginosa biofilms represents the leading cause of morbidity in cystic fibrosis
(CF). We report a precision phage therapy protocol utilizing a cocktail of four lytic bacteriophages (ΦF1-ΦF4) selected
based on patient-specific bacterial genotyping and CRISPR-spacer analysis. In a 24-month prospective cohort of 45
CF patients with multidrug-resistant infections, nebulized phage administration reduced bacterial load by 3.5 log₁₀
CFU/mL and improved forced expiratory volume (FEV1) by 18%. Whole-genome sequencing confirmed minimal
phage resistance evolution, attributed to cocktail synergy and host immune modulation. This individualized
phagetherapeutic framework offers a paradigm shift in managing recalcitrant respiratory infections.